Table 21.3 describes the cleavage sites of five different restriction enzymes. After these restriction enzymes have cleaved the DNA, four of them produce sticky ends that can hydrogen bond with

Table 21.3 describes the cleavage sites of five different restriction enzymes. After these restriction enzymes have cleaved the DNA, four of them produce sticky ends that can hydrogen bond with complementary sticky ends, as shown in Figure 21.1. The efficiency of sticky ends binding together depends on the number of hydrogen bonds; more hydrogen bonds makes the ends “stickier” and more likely to stay attached. Rank these four restriction enzymes from Table 21.3 (from best to worst) with regard to the efficiency of their sticky ends binding to each other. Transcribed Image Text: TABLE 21.3
Some Restriction Enzymes Used in Gene Cloning
Restriction
Enzyme”
Sequence
Recognized’
Bacterlal Source
ВаmнI
Bacillus amyloliquefaciens H
5′-GGATCC-3′
3′-CCTAGG-5′

Sau3AI
Staphylococcus aureus 3A
5′-GATC-3′
3′-CTAG-5′
EcoRI
Escherichia coli RY13
5′-GAATTC-3′
3′-CTTAAG-5′
Nocardia aerocolonigenes
5′-GCCGGC-3′
3′-CGGCCG-5′
Nael
Pstl
Providencia stuartii
5′-CTGCAG-3′
3′-GACGTC-5′

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